Title: | Early B-cell Factor 1 Regulates Adipocyte Morphology and Lipolysis in White Adipose Tissue [ChIP-seq]
![]() |
dateReleased: |
05-22-2014
|
description: |
Examination of EBf1 binding by ChIP-seq in differentiated human adipose stromal cell (hASC) pre-adipocyte Pre-Adipocytes differentiated in-vitro were fixed in 1% formaldehyde for 15 min at room temperature and quenched for 5 min by adding glycine to a final concentration of 0.125 M. ChIP assays were then performed with custom-made EBF1 antibody or rabbit IgG . ChIP-sequencing libraries were prepared using NEBnext chip-seq library Prep master mix set from 5 ng of anti-EBF1 and anti-IgG ChIP DNA, respectively. Sequence data were generated with Illumina HiSeq 2000 single-read sequencing and aligned against the human genome (hg19, NCBI).
|
privacy: |
not applicable
|
aggregation: |
instance of dataset
|
ID: |
E-GEOD-54889
|
refinement: |
raw
|
alternateIdentifiers: |
54889
|
keywords: |
functional genomics
|
dateModified: |
06-19-2014
|
availability: |
available
|
types: |
gene expression
|
name: |
Homo sapiens
|
accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-54889/E-GEOD-54889.raw.1.zip![]() |
storedIn: |
ArrayExpress
|
qualifier: |
gzip compressed
|
format: |
TXT
|
accessType: |
download
|
authentication: |
none
|
authorization: |
none
|
accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-54889/E-GEOD-54889.processed.1.zip |
storedIn: |
ArrayExpress
|
qualifier: |
gzip compressed
|
format: |
TXT
|
accessType: |
download
|
authentication: |
none
|
authorization: |
none
|
accessURL: | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE54889 |
storedIn: |
Gene Expression Omnibus
|
qualifier: |
not compressed
|
format: |
HTML
|
accessType: |
landing page
|
primary: |
true
|
authentication: |
none
|
authorization: |
none
|
abbreviation: |
EBI
|
homePage: | http://www.ebi.ac.uk/ |
ID: |
SCR:004727
|
name: |
European Bioinformatics Institute
|
homePage: | https://www.ebi.ac.uk/arrayexpress/ |
ID: |
SCR:002964
|
name: |
ArrayExpress
|