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Title: A genome-wide approach in Arabidopsis thaliana to assess the toxicity of cadmium sulfide quantum dots      
dateReleased:
06-15-2014
description:
Cadmium sulfide quantum dots (CdS QDs) are widely used in novel equipment. The relevance of the research lies in the need to develop risk assessments for nanomaterials, using as basis a model plant species. Here a screen of Arabidopsis thaliana mutant lines was performed in an attempt to identify plants tolerant to CdS QDs. Two tolerant Ds insertion mutant lines (atnp01 and atnp02) were identified. A whole-genome microarray experiment showed how genes were regulated by CdS QDs. Most of the genes involved in the response to CdS QDs were related to detoxification and general metabolism. The two mutant lines treated with CdS QDs showed different patterns of gene expression. The genome-wide expression profile of seedlings of the two selected resistant mutants were acquired and compared to that of wild type seedlings using the Arabidopsis ATH1 Genome Array. The wild type seedlings were exposed to either 0 mg/L, 40 mg/L (1/2 MIC) or 80 mg/L (MIC wt) CdS QDs, and each of the resistant/tolerant mutant line plants to either 0 mg/L or 80 mg/L CdS QDs for 21 days.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-53989
refinement:
raw
alternateIdentifiers:
53989
keywords:
functional genomics
dateModified:
06-21-2014
availability:
available
types:
gene expression
name:
Arabidopsis thaliana
ID:
A-AFFY-2
name:
Affymetrix GeneChip Arabidopsis Genome [ATH1-121501]
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-53989/E-GEOD-53989.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-53989/E-GEOD-53989.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE53989
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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