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Title: Rhodobacter sphaeroides 2.4.1 pSurS versus pBBR under singlet oxygen stress      
dateReleased:
07-04-2014
description:
To learn more about the function of SurS, we constructed an over-expression strain. For this purpose, the surS gene together with its native RpoE promoter was cloned into the middle-copy plasmid pBBR1 and expressed in the R. sphaeroides wild-type 2.4.1 background. The corresponding strain consequently exhibits a singlet oxygen-induced SurS over-expression compared to an empty vector control. Total RNA from the over-expression (pSurS) and from the empty vector control (pBBR) strain was extracted after 7 min of singlet oxygen stress and used for microarray analysis to investigate SurS-induced changes on transcriptome level. RNA samples collected after 7 min of singlet oxygen stress were analyzed by two-color microarrays. Each microarray contains a pool of three independent biological replicates for each sample.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-59046
refinement:
raw
alternateIdentifiers:
59046
keywords:
functional genomics
dateModified:
07-15-2014
availability:
available
types:
gene expression
name:
Rhodobacter sphaeroides 2.4.1
ID:
A-GEOD-15457
name:
Klug lab - Rhodobacter sphaeroides - 15k array Version 2 (144 sRNA)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-59046/E-GEOD-59046.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-59046/E-GEOD-59046.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE59046
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress
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