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Title: Transcription profiling by high throughput sequencing of rice dexamethasone-inducible OSH1 overexpressor before and after dexamethasone treatment      
dateReleased:
08-07-2014
description:
In flowering plants, knotted1-like homeobox (KNOX) transcription factors play crucial roles in establishment and maintenance of the shoot apical meristem (SAM), from which aerial organs such as leaves, stems and flowers initiate. We report that a rice (Oryza Sativa) KNOX gene Oryza sativa homeobox1 (OSH1) represses the brassinosteroid (BR) phytohormone pathway through activation of BR catabolism genes. Inducible overexpression of OSH1 caused brassinosteroid insensitivity, whereas loss-of-function showed a BR-overproduction phenotype. Genome-wide identification of loci bound and regulated by OSH1 revealed hormonal and transcriptional regulation as the major function of OSH1. Among these targets, BR catabolism genes CYP734A2, CYP734A4 and CYP734A6 were rapidly up-regulated by OSH1-induction. Furthermore, RNAi knockdown plants of CYP734A genes arrested growth of the SAM and mimicked some osh1 phenotypes. Thus, we suggest that local control of BR levels by KNOX genes is a key regulatory step in SAM function.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-4316
refinement:
raw
keywords:
functional genomics
dateModified:
01-19-2016
creators:
Sarah Hake
availability:
available
types:
gene expression
name:
Oryza sativa Japonica Group
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-4316/E-MTAB-4316.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-4316/E-MTAB-4316.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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