Mountain View
biomedical and healthCAre Data Discovery Index Ecosystem
help Advanced Search
Title: Global gene expression analysis reveals importance of thermal effect in cellular response to millimeter wave exposure      
dateReleased:
08-12-2014
description:
To evaluate the effect of millimeter waves (MMW) exposure on the gene expression, we have employed whole genome microarray expression profiling. Neonatal primary Keratinocyte cells pooled from 3 healthy donors were exposed ex vivo, 3 hours at 20 mW/cm². At this condition, corresponding to the maximum power density authorized for public use, MMW exposure induces a significant increase of the temperature and 665 genes were found differentially expressed. To determine the role of hyperthermia in this response, several controls were performed. When the temperature artificially maintained constant, no specific gene modifications were observed after exposure. However, a heat shock control did not mimic exactly the MMW effect: 34-gene signatures were identified between 20 mW/cm2 MMW exposure and its relative heat shock control. Differential expression of seven genes (ADAMTS6, NOG, IL7R, FADD, JUNB, SNAI2 and HIST1H1A) from this signature were confirmed by duplication of the whole experiment and real-time PCR analysis. Gene expression in human keratinocytes was measured after a 60 GHz-exposure at 20 mW/cm2, for 3 h (Expo). It was compared to cells cultured in unexposed condition (Sham). As millimeter wave (MMW) exposure increase temperature in the cell medium, two controls were performed: 1) MMW exposure (3 h, 60 GHz, 20 mW/cm²) with the heat increase compensated to maintain constant the physiological temperature (CompT_Expo); 2) Heat shock control (HSC) to mimicking the heat effect of MMW exposure.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-57135
refinement:
raw
alternateIdentifiers:
57135
keywords:
functional genomics
dateModified:
08-15-2014
availability:
available
types:
gene expression
name:
Homo sapiens
ID:
A-GEOD-14550
name:
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Probe Name Version)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-57135/E-GEOD-57135.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-57135/E-GEOD-57135.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE57135
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress
Similar Datasets

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on GitHub.