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Title: Integration of growth and patterning during vascular tissue formation in Arabidopsis      
dateReleased:
08-15-2014
description:
Coordination of cell division and pattern formation is central to tissue and organ development, and is particularly important in plants where walls prevent cell migration. Auxin and cytokinin are both critical for division and patterning, but it is unknown how these hormones converge to control tissue development. Here, we identify a genetic network that reinforces an early embryonic bias in auxin distribution to create a local, non-responding cytokinin source within the root vascular tissue. We provide experimental and theoretical evidence that these cells act as a local tissue organizer by positioning the domain of oriented cell divisions. We further demonstrate that the auxin-cytokinin interaction acts as a spatial incoherent feed forward loop, which is essential to generate distinct hormonal response zones, thus establishing a stable pattern within a growing vascular tissue. 4-day-old plants grown on MS were treated with the indicated compounds for the indicated time, whereafter roots were harvested and total RNA was isolated. Alternatively, roots were protoplasted and cell sorted for GFP+ cells followed by total RNA extraction.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-56868
refinement:
raw
alternateIdentifiers:
56868
keywords:
functional genomics
dateModified:
08-26-2014
availability:
available
types:
gene expression
name:
Arabidopsis thaliana
ID:
A-GEOD-17416
name:
[AraGene-1_0-st] Arabidopsis Gene 1.0 ST Array [transcript (gene) version]
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-56868/E-GEOD-56868.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-56868/E-GEOD-56868.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE56868
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress
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