biomedical and healthCAre Data Discovery Index Ecosystem
biomedical and healthCAre Data Discovery Index Ecosystem
| Title: | Integrative AUF1 PAR-CLIP analysis uncovers AUF1 roles in translation and genome integrity (RNA-Seq 1)
|
| dateReleased: |
11-04-2014
|
| description: |
We report that AUF1 modulates global mRNA stability and translation, in turn promoting the maintenance of DNA integrity. Please see individual series. For AUF1 PAR-CLIP, the four isoforms of AUF1 (p37, p40, p42, and p45) tagged with a Flag epitope were expressed in HEK293 cells. For total RNA-Seq HEK293 cells were transfected with Control siRNA, AUF1 siRNA, Empty Vector, Flag-AUF1 p37, p40, p42, or p45 as well as WI-38 cells were collected at PDL 15 and 55 and also transfected with Control siRNA, AUF1 siRNA, HuR siRNA. For Ribo-Seq HeLa cells were transfected with Control siRNA, AUF1 siRNA, or HuR siRNA.
|
| privacy: |
not applicable
|
| aggregation: |
instance of dataset
|
| ID: |
E-GEOD-52973
|
| refinement: |
raw
|
| alternateIdentifiers: |
52973
|
| keywords: |
functional genomics
|
| dateModified: |
12-23-2014
|
| availability: |
available
|
| types: |
gene expression
|
| name: |
Homo sapiens
|
| accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-52973/E-GEOD-52973.raw.1.zip |
| storedIn: |
ArrayExpress
|
| qualifier: |
gzip compressed
|
| format: |
TXT
|
| accessType: |
download
|
| authentication: |
none
|
| authorization: |
none
|
| accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-52973/E-GEOD-52973.processed.1.zip |
| storedIn: |
ArrayExpress
|
| qualifier: |
gzip compressed
|
| format: |
TXT
|
| accessType: |
download
|
| authentication: |
none
|
| authorization: |
none
|
| accessURL: | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE52973 |
| storedIn: |
Gene Expression Omnibus
|
| qualifier: |
not compressed
|
| format: |
HTML
|
| accessType: |
landing page
|
| primary: |
true
|
| authentication: |
none
|
| authorization: |
none
|
| abbreviation: |
EBI
|
| homePage: | http://www.ebi.ac.uk/ |
| ID: |
SCR:004727
|
| name: |
European Bioinformatics Institute
|
| homePage: | https://www.ebi.ac.uk/arrayexpress/ |
| ID: |
SCR:002964
|
| name: |
ArrayExpress
|