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Title: The quantitative genetics of transcriptional variation in stickleback liver      
dateReleased:
12-03-2014
description:
Total RNA was extracted from liver tissues of lab-reared threespine stickleback. First generation fish originating from the Baltic Sea near Helsinki (Finland) were bred using a paternal half-sib design. At 6 months individuals were randomly assigned to either a treatment or control group: the temperature of treated fish (T) was raised 1oC per hour over the course of 6h to a final temperature of 23oC, then maintained for 1h at final temperature; control (C) fish were maintained at 17oC under similar holding conditions. Immediately following experimental (or sham) treatment, fish were euthanized by anaesthetic overdose. Liver tissues were immediately dissected and flash frozen in liquid nitrogen for subsequent RNA extraction. Annotated R scripts defining the normalization procedures are available as additional files (see ). Additional files also include a targets file to assist with reading raw data into R (Targs.txt), and a metadata file (matrix.df.csv) to facilitate construction of design matrices used by the âsnmâ package.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-3098
refinement:
raw
dateSubmitted:
12-31-2008
keywords:
functional genomics
dateModified:
12-08-2014
availability:
available
types:
gene expression
name:
Gasterosteus aculeatus
name:
family based design
ID:
A-MTAB-532
name:
Threespine Stickleback Agilent GE 8x15K v1
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3098/E-MTAB-3098.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3098/E-MTAB-3098.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress