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Title: Comparison of gene expression of WT and PerM mutant M. tuberculosis H37Rv grown in high and reduced magnesium      
dateReleased:
01-05-2015
description:
M. tuberculosis membrane protein PerM is required for persistence in chronic mouse infection. In vitro, the perM mutant requires increased magnesium for replication and survival, with elongation and lysis in low-magnesium media. To gain insight into the function of PerM, we compared the transcriptomes of wt and perM mutant Mtb grown in high (2000 μM) and reduced (250 μM) Mg2+ in three independent experiments. Transcriptome analysis of the perM mutant grown in reduced magnesium revealed upregulation of cell division and cell wall biosynthesis genes. Bacteria were grown for 5 days in Sauton’s media containing 250 or 2000 μM MgCl2 and 0.05% Tween 80. Three independent experiments were performed.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-57263
refinement:
raw
alternateIdentifiers:
57263
keywords:
functional genomics
dateModified:
01-10-2015
availability:
available
types:
gene expression
name:
Mycobacterium tuberculosis
ID:
A-GEOD-16177
name:
Agilent-019943 Genotypic Technology custom M tuberculosis h37Rv Whole Genome 8x15k GE Microarray [Gene Name version]
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-57263/E-GEOD-57263.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-57263/E-GEOD-57263.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE57263
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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