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Title: Transcription profiling by array of fracture callus obtained from rats treated with sponges loaded either with GDF5wt or GDF5N445T and sacrificed at day 3, 5, 7 and 14 post-operation      
dateReleased:
01-15-2015
description:
Seventy-two female Sprague-Dawley rats underwent a 5 mm critical-sized femoral segmental defect followed by stabilization with a custom-made unilateral external fixator. Following femoral defect generation, absorbable type I bovine collagen sponges were loaded either with 50 ug GDF5wt or 50 ug GDF5N445T, dissolved in dilution buffer (10 mM HCl), and placed into the defect. The control group was treated with collagen sponges soaked with dilution buffer. For analysis of the early healing phase, 48 rats (n = 4 rats/group & time point) were sacrificed at day 3, 5, 7 and 14 post-operation and the callus tissue between the two inner pins was harvested and immediately flash frozen in liquid nitrogen for RNA extraction. Tissue was subsequently pulverized and total RNA was isolated using Trifast reagent and RNeasy Mini Kit. The total RNA from 3 animals per group and time point were pooled prior to hybridization on an Affymetrix GeneChip Rat Gene 1.0 ST Array.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-2249
refinement:
raw
keywords:
functional genomics
dateModified:
01-14-2015
creators:
Claus E Ott
availability:
available
types:
gene expression
name:
Rattus norvegicus
ID:
A-AFFY-160
name:
Affymetrix GeneChip Rat Gene 1.0 ST Array [RaGene-1_0-st-v1]
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-2249/E-MTAB-2249.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-2249/E-MTAB-2249.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress