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Title: Candidatus pelagibacter ubique HTCC1062 requires 4-amino-5-hydroxymethyl-2-methylpyrimidine, an abundant thiamine precursor in the sea      
dateReleased:
01-20-2015
description:
Thiamine is often undetectable in ocean surface waters where Pelagibacter cells are numerically abundant. Despite this, Pelagibacter cells are missing de novo thiamine synthesis pathways. We show that an eogenous source of the thiamine precursor HMP is required for thiamine synthesis in Pelagibacter and that this precursor is abundant in the Sargasso sea. Batch cultures of P. ubique were grown in a defined arificial seawater media. Three cultures were given no thiamine amendment, and three other cultures received an excess concentration of thiamine. Cultures were harvested for microarray analyses just prior to and after thiamine limitation for the purpose of observing differences in gene expression related to thiamine limitation.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-46673
refinement:
raw
alternateIdentifiers:
46673
keywords:
functional genomics
dateModified:
01-27-2015
availability:
available
types:
gene expression
name:
Candidatus Pelagibacter ubique HTCC1062
ID:
A-GEOD-7330
name:
Candidatus Pelagibacter ubique OSU_SAR11_affychip
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-46673/E-GEOD-46673.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-46673/E-GEOD-46673.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE46673
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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