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Title: Detection of differential gene expression in locust brain infected with the protozoan parasite Neospora caninum using the drosophila gene chip      
dateReleased:
01-28-2015
description:
To obtain a broader understanding of the effects of the N. caninum on S. gregaria adults, we examined the parasite impact on host gene expression using Affymetrix DNA microarray and Cross Species Hybridisation (CSH) analysis. Since N. caninum infection induced neurological injuries in locusts, it was important to test whether N. caninum infection had altered gene expression within locust brains. The analysis was designed to gain further understanding of the mechanisms for N. caninum neuropathy, and in particular, of the genes responsible for the capacity of N. caninum to establish brain infection. Using a cross species hybridisation (CSH) approach, RNA samples from brains of mock- and N. caninum-infected locusts were labeled and hybridised to Affymetrix Gene Chip Drosophila Genome 2.0 Arrays. Data were analysed using Partek Genomics Suite Version 6.4. Of the 28,593 transcripts represented on the GeneChip, approximately 18,500 were expressed in the N. caninum-infected locust’s brain. PCA quality control analysis shows that the uninfected and infected samples were biologically noisy but generally well separated .
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-3132
refinement:
raw
dateSubmitted:
12-03-2013
keywords:
functional genomics
dateModified:
11-28-2014
creators:
Hany M Elsheikha
availability:
available
types:
gene expression
name:
Locusta migratoria
name:
disease state design
ID:
A-AFFY-35
name:
Affymetrix GeneChip Drosophila Genome 2.0 Array [Drosophila_2]
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3132/E-MTAB-3132.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3132/E-MTAB-3132.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress
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