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Title: Real-time quantitative PCR analysis of the expression of cytoskeleton regulator genes in 2 week old male MeCP2 A140V mutant mouse brain cortex      
dateReleased:
02-01-2015
description:
The goal of this study was to identify brain cortex gene expression differences between mutant MeCP2 A140V mice and their wild type littermates.The PCR array used for these experiments was the Mouse Cytoskeleton Regulators Array (Catalog# PAMM-088A) from SABiosciences. Mice used in this study were 2 week old males. Total RNA was extracted from the somatosensory/motor cortex of 2 week old male MeCP2 A140V hemizygous mutant mice and their wild type littermates. For this study, cortical tissue from 3 mice of each genotype was used and RNA from the cortex of each mouse was analyzed separately (ie., triplicate biological replicates). The RNA was purified to remove genomic DNA and was then reverse transcribed for use in quantitative RT-PCR analysis of gene expression using the SABiosciences RT2 profiler PCR array system. The PCR array used for these experiments was the Mouse Cytoskeleton Regulators Array (Catalog# PAMM-088A).
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-35769
refinement:
raw
alternateIdentifiers:
35769
keywords:
functional genomics
dateModified:
02-08-2015
availability:
available
types:
gene expression
name:
Mus musculus
ID:
A-GEOD-15213
name:
SABiosciences Mouse Cytoskeleton Regulators Array (PAMM-088A)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-35769/E-GEOD-35769.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-35769/E-GEOD-35769.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE35769
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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