dateReleased: |
02-27-2015
|
description: |
The general objective of the study was to determine modulation of gene expression by environmental factors, with a special emphasis on bone formation. For this reason, the specific period of treatment was chosen between 5-6 days post-fertilization (dpf), when bone formation and calcification are taking place. This experiment was designed as a new type of gravitational experiment, which we like to call \relative microgravity\, referring to the fact that the larvae first grow in hyper gravity for 5 days and are then returned to 1g normal gravity for 1 day. Zebrafish embryos were placed on a Large Diameter Centrifuge at 3 hpf, brought to a gravitational force of 3 g until 5 dpf. Reference embryos were kept in parallel at 1g (Inc). At 5dpf, one batch was left at 3g (3g), one batch was returned to 1g (3g>1g), while a third batch was returned to 1g, but left on the axis of the centrifuge (Axe; 3g>Axe). The experiment was repeated 4 times, each time with 4 batches of 60 larvae.
|
privacy: |
not applicable
|
aggregation: |
instance of dataset
|
ID: |
E-MTAB-3290
|
refinement: |
raw
|
dateSubmitted: |
10-21-2010
|
keywords: |
functional genomics
|
dateModified: |
02-02-2015
|
availability: |
available
|
types: |
gene expression
|
name: |
Danio rerio
|
name: |
stimulus or stress design
|
ID: |
A-GEOD-10816
|
name: |
Agilent-024129 Dano rerio 44K
|
accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3290/E-MTAB-3290.raw.1.zip |
storedIn: |
ArrayExpress
|
qualifier: |
gzip compressed
|
format: |
TXT
|
accessType: |
download
|
authentication: |
none
|
authorization: |
none
|
accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3290/E-MTAB-3290.processed.1.zip |
storedIn: |
ArrayExpress
|
qualifier: |
gzip compressed
|
format: |
TXT
|
accessType: |
download
|
authentication: |
none
|
authorization: |
none
|
abbreviation: |
EBI
|
homePage: | http://www.ebi.ac.uk/ |
ID: |
SCR:004727
|
name: |
European Bioinformatics Institute
|
homePage: | https://www.ebi.ac.uk/arrayexpress/ |
ID: |
SCR:002964
|
name: |
ArrayExpress
|