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Title: Transcription profiling by array of zebrafish larvae exposed to relative microgravity      
dateReleased:
02-27-2015
description:
The general objective of the study was to determine modulation of gene expression by environmental factors, with a special emphasis on bone formation. For this reason, the specific period of treatment was chosen between 5-6 days post-fertilization (dpf), when bone formation and calcification are taking place. This experiment was designed as a new type of gravitational experiment, which we like to call \relative microgravity\, referring to the fact that the larvae first grow in hyper gravity for 5 days and are then returned to 1g normal gravity for 1 day. Zebrafish embryos were placed on a Large Diameter Centrifuge at 3 hpf, brought to a gravitational force of 3 g until 5 dpf. Reference embryos were kept in parallel at 1g (Inc). At 5dpf, one batch was left at 3g (3g), one batch was returned to 1g (3g>1g), while a third batch was returned to 1g, but left on the axis of the centrifuge (Axe; 3g>Axe). The experiment was repeated 4 times, each time with 4 batches of 60 larvae.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-3290
refinement:
raw
dateSubmitted:
10-21-2010
keywords:
functional genomics
dateModified:
02-02-2015
availability:
available
types:
gene expression
name:
Danio rerio
name:
stimulus or stress design
ID:
A-GEOD-10816
name:
Agilent-024129 Dano rerio 44K
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3290/E-MTAB-3290.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3290/E-MTAB-3290.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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