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Title: hiCLIP analysis of RNA duplexes bound by STAU1 in HEK293 cells      
dateReleased:
03-02-2015
description:
hiCLIP protocol was followed to isolate RNA bound to 3XFLAG-STAU1 protein and amplify cDNA library as described in the manuscript. Specific barcodes were used for each RT reaction, and are specified below for each sequencing run. Random barcodes were also incoperated into cDNAs to distinguish between PCR duplication of cDNA products. The final products were obtained by PCR with Illumina paired-end sequencing primers: 5-CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT-3; 5-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-2937
refinement:
raw
keywords:
functional genomics
dateModified:
03-20-2015
creators:
Jernej Ule
availability:
available
types:
gene expression
name:
Homo sapiens
name:
binding site identification design
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-2937/E-MTAB-2937.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-2937/E-MTAB-2937.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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