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Title: A Floor Plate Extracellular Protein-Protein Interaction Screen Identifies Draxin as a Secreted Netrin-1 Antagonist      
dateReleased:
05-31-2015
description:
Floor plate-derived extracellular signaling molecules, including canonical axon guidance cues of the Netrin family, control neuronal circuit organization. Despite the importance of the floor plate as an essential signaling centre in the developing vertebrate central nervous system, no systematic approach to identify binding partners for floor plate-expressed cellsurface and secreted proteins has been carried out. Here, we used a high-throughput assay to discover extracellular protein-protein interactions, which likely take place in the zebrafish floor plate microenvironment. The assembled floor plate network contains 47 interactions including the hitherto not reported interaction between Netrin-1 and Draxin. We further characterized this interaction, narrowed down the binding interface, and demonstrated that Draxin competes with Netrin receptors for binding to Netrin-1. Our results suggest that Draxin functions as an extracellular Netrin signaling modulator in vertebrates. A reciprocal gradient of Draxin might shape or sharpen the active Netrin gradient, thereby critically modulating its effect.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-3609
refinement:
raw
dateSubmitted:
03-11-2010
keywords:
functional genomics
dateModified:
05-28-2015
creators:
Mitchell Levesque
availability:
available
types:
gene expression
name:
Danio rerio
name:
genetic modification design
ID:
A-MEXP-1926
name:
Agilent D. rerio (Zebrafish) Oligo Microarray 4x44K 019161 G2519F v2 (266 cols x 170 rows)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3609/E-MTAB-3609.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3609/E-MTAB-3609.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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