Mountain View
biomedical and healthCAre Data Discovery Index Ecosystem
help Advanced Search
Title: Wnt directs the endosomal flux of LDL-derived cholesterol and lipid droplet homeostasis      
dateReleased:
06-20-2015
description:
The Wnt pathway, which controls crucial steps of the development and differentiation programs, has been proposed to influence lipid storage and homeostasis. In this paper, using an unbiased strategy based on high content genome-wide RNAi screens that monitored lipid distribution and amounts, we find that Wnt3a regulates cellular cholesterol. We show that Wnt3a stimulates the production of lipid droplets, and that this stimulation strictly depends on endocytosed, LDL-derived cholesterol and on functional early and late endosomes. We also show that Wnt signaling itself controls cholesterol endocytosis and flux along the endosomal pathway, which in turn modulates cellular lipid homeostasis. These results underscore the importance of endosome functions for LD formation and reveal a previously unknown cellular program controlling lipid storage and endosome transport under the control of Wnt signaling.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-2872
refinement:
raw
dateSubmitted:
08-24-2014
keywords:
functional genomics
dateModified:
06-20-2015
availability:
available
types:
gene expression
name:
Homo sapiens
name:
growth condition design
ID:
A-GEOD-17889
name:
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [HuGene20stv1_Hs_ENTREZG_16.0.0]
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-2872/E-MTAB-2872.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-2872/E-MTAB-2872.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on GitHub.