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Title: Human Calu-3 cell transcriptome response to human interferon α [miRNA]      
dateReleased:
06-25-2015
description:
The purpose is to obtain samples for mRNA and miRNA analysis with and without interferon α treatment; in parallel, to obtain cell pellets to forward to PNNL for use in top-down proteomics pilot experiments Human Calu-3 cells were seeded at 1 x 10^6 cells per well on 6 well plates 3 days before infection. Treatments were initiated by replacing culture medium (containing 10% FBS) with fresh medium containing 500 U/ml of human interferon α (PBL Biosciences). Mock-treated cells were given normal culture medium only. Mock and treated cells were incubated at 37°C until sample collection time points. Infected samples were collected in quadruplet; time-matched mocks were collected in quadruplet in parallel with infected samples. Time points: 6, 12, and 18 h post-infection
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-70220
refinement:
raw
alternateIdentifiers:
70220
keywords:
functional genomics
dateModified:
06-26-2015
availability:
available
types:
gene expression
name:
Homo sapiens
ID:
A-GEOD-19730
name:
Agilent-046064 Unrestricted_Human_miRNA_V19.0_Microarray (Probe Name version)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-70220/E-GEOD-70220.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-70220/E-GEOD-70220.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE70220
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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