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Title: Inefficient translation of nsrR constrains behavior of the NsrR regulon in Escherichia coli      
dateReleased:
06-27-2015
description:
Investigation of whole genome gene expression level changes in a Escherichia coli MG1655 K-12 nsrR with AUG start codon compared to the wild type nsrR (with a GUG start codon) and to the control lacking the nsrR gene. Conversion of the nsrR start codon from the wild type GUG to AUG increased the efficiency of translation and had measurable effects on the expression patterns of some NsrR regulated genes. A nine chip study using total RNA recovered from three separate cultures of Escherichia coli MG1655 K-12 AUGnsrR, three separate cultures of the WT nsrR (GUGnsrR) and three separate cultures of nsrR deletion strain. Each chip measures the expression level of 4,254 genes from Escherichia coli MG1655 K-12 with eight 60-mer probes per gene, with 2-fold technical redundancy.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-70325
refinement:
raw
alternateIdentifiers:
70325
keywords:
functional genomics
dateModified:
08-20-2015
availability:
available
types:
gene expression
name:
Escherichia coli str. K-12 substr. MG1655
ID:
A-GEOD-14649
name:
071112 Ecoli K12 EXP X4
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-70325/E-GEOD-70325.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-70325/E-GEOD-70325.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE70325
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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