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Title: Transcriptome profiling of single zebrafish cells      
dateReleased:
07-13-2015
description:
Individual WT zebrafish cells were placed into lysis buffer containing a set quantity of ERCC spikes. RNA within the lysis buffer was chemically fragmented and the 3' ends of RNA was pulled down from the lysis buffer using polyT oligos attached to magnetic beads. RNA oligo comprising part of the Illumina adapter 2 was ligated to the 5 end of the captured RNA and the RNA was eluted from the beads. Reverse transcription was primed with an anchored polyT oligo with part of Illumina adapter 1 at the 5 end followed by 12 random bases, then an 8 base indexing tags, then CG and 14 T bases. An Illumina library with full adapter sequence was produced by 30 cycles of PCR. This data has specifically been generated to study mRNA levels in single cells. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-ERAD-412
refinement:
raw
keywords:
functional genomics
dateModified:
05-18-2016
creators:
The Wellcome Trust Sanger Institute
availability:
available
types:
gene expression
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-ERAD-412/E-ERAD-412.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-ERAD-412/E-ERAD-412.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress