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Title: Remodeling of DNA transcription genomic fabric in Capridine-treated LNCaP human prostate cancer cell line      
dateReleased:
08-27-2015
description:
Capridine is a chemotherapeutic agent with specificity towards prostate cancer and minimal bone marrow toxicity. Capridine structure is consistent with a classical DNA intercalating agent, although its DNA intercalation property does not fully correlate with its anti prostate cancer specific activity. We have used Agilent human gene expression arrays to identify the cellular targets of Capridine on androgen responsive LNCaP cells, a classical cell lines for prostate cancer studies. We found that expression of 142 unigenes genes was altered by Capridine. Interestingly, the most altered category was that of transcription regulators: 10 down and 16 upregulated. Our data confirm that, in addition to DNA intercalation, Capridine targets DNA transcription via histone modulation in a cell-specific manner. Two-conditions: LO = not treated, LA = Capridine-treated DU145 cells. Four biological replicates of each condition. Differently labeled biological replicates were cohybridized with each array. Results of similarly labeled different conditions were compared then averaged for the two fluorescent labels. For instance: LO1 & LO3 were compared with LA1 & LA3, LO2 & LO4 were compared with LA2 & + LA4, and the results of the comparisons averaged. This design uses 100% of the resources, has a better normlaiztion and allows all possible comparisons among the conditions.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-72414
refinement:
raw
alternateIdentifiers:
72414
keywords:
functional genomics
dateModified:
09-06-2015
availability:
available
types:
gene expression
name:
Homo sapiens
ID:
A-GEOD-10332
name:
Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Feature Number version)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-72414/E-GEOD-72414.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-72414/E-GEOD-72414.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE72414
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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