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Title: On the relevance of technical variation due to building pools in microarray experiments      
dateReleased:
09-10-2015
description:
The objective of the study was to assess the technical error due to blending of individual samples into pools in experimental data. The blending error variance component corresponds to random effects for inaccuracies, which were modeled on the logarithmic scale of normalized gene expression. It's estimation based on a linear mixed model, fitted for each transcript. Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the metestrous stage. They were combined to 6 pools of 5 individuals each and processed for microarray analysis. Additionally, the 10 individuals used for the first two pools of each line were processed.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-72861
refinement:
raw
alternateIdentifiers:
72861
keywords:
functional genomics
dateModified:
09-11-2015
availability:
available
types:
gene expression
name:
Mus musculus
ID:
A-AFFY-23
name:
Affymetrix GeneChip Mouse Expression Array MOE430A [MOE430A]
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-72861/E-GEOD-72861.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-72861/E-GEOD-72861.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE72861
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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