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Title: Gene expression analysis of H358 non-small cell lung cancer cells selected for resistance against erlotinib or wild-type      
dateReleased:
09-15-2015
description:
Erlotinib is a tyrosine kinase inhibitor (TKI) that is approved as a second-line monotherapy in patients with advanced non-small cell lung cancer (NSCLC). In these patients, erlotinib prolongs survival but its benefit remains modest since overtime, many tumors develop resistance. To analyse the changes in the gene expression profile, that accompany resistance development, we treated the erlotinib sensitive non-small cell lung cancer cell line H358 with increasing concentrations of erlotinib (1-5µM) for several weeks (H358res). In parallel, we kept H358 with the same concentrations of the vehicle DMSO (H358co). After ten weeks of treatment, when the H358res stably grew under 5µM erlotinib, total RNA of both cell lines was harvested and hybridized.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-3387
refinement:
raw
dateSubmitted:
07-15-2010
keywords:
functional genomics
dateModified:
03-09-2015
availability:
available
types:
gene expression
name:
Homo sapiens
name:
stimulus or stress design
ID:
A-GEOD-4454
name:
Affymetrix GeneChip Human Genome U133 Plus 2.0 Array based on a custom CDF
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3387/E-MTAB-3387.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3387/E-MTAB-3387.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress