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Title: Phasevarion of non-typeable Haemophilus influenzae R2866      
dateReleased:
09-24-2015
description:
H. influenzae R2866 contains a phase-variable DNA methylase modA10, by virtue of 16 tetrameric repeats present after the start codon. Gain or loss of repeats during replication by slipped-strand mispairing can result in a non-functional reading frame. Microarray analyses of wild-type and insertionally inactivated modA were performed to determine genes affected by phase variable modification. Three replicate cultures were grown in either supplemented brain heart infusion broth (R2866 and R3763) or chemically defined media (R3790 and R3791) to an optical density at 600nm of 0.3 to 0.4 (mid-log phase). RNA was isolated by the method of Jahn et al.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-73349
refinement:
raw
alternateIdentifiers:
73349
keywords:
functional genomics
dateModified:
09-25-2015
availability:
available
types:
gene expression
name:
Haemophilus influenzae R2866
ID:
A-GEOD-6594
name:
Nimblegen custom Haemophilus influenzae multi-genome array
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-73349/E-GEOD-73349.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-73349/E-GEOD-73349.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE73349
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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