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Title: Mouse Dendritic Cells Expression Data      
dateReleased:
10-24-2015
description:
Dendritic cells (DC) play a vital role in the induction of activation or tolerance of immune response. Histone deacetylase (HDAC) inhibitors potently modulate experimental graft-vs-host disease, allograft rejection, and autoimmune diseases, partly through the regulation of dendritic cells. The molecular mechanisms underpinning their immunosuppressive effects on DCs are not well understood. The functional relevance of acetylation in DC responses remains undefined. We used microarrays to detail the global program of gene expression in dendritic cells after treated with or without SAHA, a HDAC inhibitor utilized in clinic, to identify the upregulated and downregulated genes. To obtain overall gene expression profile, we extracted bone marrow cells from at least 3 mice for each experiment. Purified (95% purity) mouse dendritic cells were treated with SAHA (500 nm for 12 hours) or control diluent, then used for RNA extraction and hybridization on Affymetrix microarrays.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-74306
refinement:
raw
alternateIdentifiers:
74306
keywords:
functional genomics
dateModified:
11-24-2015
availability:
available
types:
gene expression
name:
Mus musculus
ID:
A-AFFY-45
name:
Affymetrix GeneChip Mouse Genome 430 2.0 [Mouse430_2]
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-74306/E-GEOD-74306.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-74306/E-GEOD-74306.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE74306
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress