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Title: RNA-seq analysis to investigate the downstream genes of EXB1 in Arabidopsis      
dateReleased:
12-21-2015
description:
Arabidopsis EXCESSIVE BRANCHES 1 (EXB1) affects both axillary meristem (AM) initiation and bud activities. To investigate the downstream genes of EXB1, the construct 4EnhpEXB1-EXB1GR in which EXB1 was fused to the sequence encoding the steroid-binding domain of the rat glucocorticoid receptor (GR) was constructed and EXB1 was driven by four CaMV 35S enhancers and EXB1 promoter. We performed RNA-seq analysis on the tissues around leaf axils of 14-day-old 4EnhpEXB1-EXB1GR-13 transgenic lines treated with or without DEX. The RNA-seq results showed that 3114 genes were differentially expressed, with 1015 up-regulated and 2099 down-regulated. Among these genes, three MYB family transcription factor genes, i.e., RAX1, RAX2 and RAX3, known to regulate axillary meristem initiation were identified suggesting that RAX1, RAX2 and RAX3 are probably downstream genes of EXB1.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-4002
refinement:
raw
dateSubmitted:
10-21-2015
keywords:
functional genomics
dateModified:
11-03-2015
creators:
Genji Qin
availability:
available
types:
gene expression
name:
Arabidopsis thaliana
name:
genetic modification design
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-4002/E-MTAB-4002.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-4002/E-MTAB-4002.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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