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Title: Transcriptomic impact of AWR5 effector expression in S. cerevisiae      
dateReleased:
12-31-2015
description:
The type III effector (T3E) AWR5 from the phytopathogen Ralstonia solanacearum causes disease in many plants relevant in agriculture. Heterologous expression in yeast of AWR5 from an integrated tetO promoter caused strong growth inhibition. In this series of experiments we investigate the possible changes in the transcriptional profile in a time-course (2, 4 and 6 h) response to AWR5 production. Yeast strains were grown overnight in rich YPD medium with doxycycline 15 µg/ml (repressing conditions), then normalized to OD600=0.05 and grown resumed in YPD+dox (non-inducing conditions) or YPD (inducing conditions). Samples were taken at 2, 4 or 6 hours for each condition. Two biological replicates were made for each time point.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-70202
refinement:
raw
alternateIdentifiers:
70202
keywords:
functional genomics
dateModified:
01-18-2016
availability:
available
types:
gene expression
name:
Saccharomyces cerevisiae
ID:
A-GEOD-10039
name:
Yeast_16p_DNA_Feb_2010
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-70202/E-GEOD-70202.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-70202/E-GEOD-70202.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE70202
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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