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Title: Transcriptional profiling by array of Solenopsis invicta (fire ant) newly mated queens to identify genes and gene pathways responding to infection by SINV viruses      
dateReleased:
01-04-2016
description:
We performed a whole-genome transcriptomic analysis of pleometrotic queens infected by SINV-1, SINV-2 or co-infected with both viruses, to characterize patterns of gene expression associated with viral infection and identify genes responding differentially to the two viruses. We sampled fire ant queens in Gainesville, Florida, immediately after a mating flight. We arranged them in pairs based on having similar weights (range ±0.2 mg) to allow pleometrotic colony founding. We housed paired queens in nesting tubes in claustral conditions (no food or water) for one month. Thereafter, we collected queens in dry ice, we isolated total RNA from whole bodies and used it to quantify SINV-1 and SINV-2 viruses with real-time PCR. We selected 2 queens that were virus-free as controls, 3 SINV-1-infected, 4 SINV-2-infected and 11 co-infected by both viruses. These samples were processed for microarrays analysis.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-3581
refinement:
raw
dateSubmitted:
12-12-2012
keywords:
functional genomics
dateModified:
01-04-2016
creators:
Fabio Manfredini
availability:
available
types:
gene expression
name:
Solenopsis invicta
ID:
A-MEXP-2297
name:
GrozingerLab S.invicta 12-plex-135k pleo1
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3581/E-MTAB-3581.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3581/E-MTAB-3581.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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