biomedical and healthCAre Data Discovery Index Ecosystem
biomedical and healthCAre Data Discovery Index Ecosystem
| Title: | Genetic variability exceeds cell-type memory and determines iPSCs differentiation potential-implications for biobanking
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| dateReleased: |
01-07-2016
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| description: |
Summary To address the issue of retention of somatic cell memory in iPSCs we compared gene expressions of genetically matched human iPSCs derived from fibroblasts and blood. We were using the all-in-one type footprint free SeVdp-iPS system, for generation of uniform iPSC lines Our results show that iPSC lines derived from the same donor are highly similar to each other. Overal design Total RNA was extracted from fibroblasts derived iPSC (N=8) and blood derived iPSC (N=10) as well as parental fibroblasts and peripheral blood mononuclear cells (N=4 different donors: T14; T42; T53; T55). Total RNA was also extracted from spontaneously differentiated Embryonic bodies derived from fibroblasts derived iPSC (N=4) and blood derived iPSC (N=4) from four donors. RNA from control human embryonic stem cell lines (H9, and FES22) was used as control. Samples were run in two different batches (1 and 2).
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| privacy: |
not applicable
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| aggregation: |
instance of dataset
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| ID: |
E-MTAB-3825
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| refinement: |
raw
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| dateSubmitted: |
07-16-2013
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| keywords: |
functional genomics
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| dateModified: |
08-22-2016
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| creators: |
Ras Trokovic
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| availability: |
available
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| types: |
gene expression
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| name: |
Homo sapiens
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| name: |
cell type comparison design
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| ID: |
A-MEXP-2072
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| name: |
Illumina HumanHT-12_V4_0_R2_15002873_B
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| accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3825/E-MTAB-3825.raw.1.zip |
| storedIn: |
ArrayExpress
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| qualifier: |
gzip compressed
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| format: |
TXT
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| accessType: |
download
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| authentication: |
none
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| authorization: |
none
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| accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3825/E-MTAB-3825.processed.1.zip |
| storedIn: |
ArrayExpress
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| qualifier: |
gzip compressed
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| format: |
TXT
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| accessType: |
download
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| authentication: |
none
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| authorization: |
none
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| abbreviation: |
EBI
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| homePage: | http://www.ebi.ac.uk/ |
| ID: |
SCR:004727
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| name: |
European Bioinformatics Institute
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| homePage: | https://www.ebi.ac.uk/arrayexpress/ |
| ID: |
SCR:002964
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| name: |
ArrayExpress
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