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Title: Transcription profiling by array of Saccharomyces cerevisiae in response to ethanol      
dateReleased:
01-12-2016
description:
The genome-wide transcriptional responses of the wild type laboratory strain, and two deletion strains (pmt7Δ/pmt7Δ and yhl042wΔ/yhl042wΔ) were comparatively investigated in the absence and in the presence of ethanol for two hours. Strains were cultivated in flasks at 30°C and 180 rpm in an orbital shaker till the mid-exponential phase of growth (OD600 of 0.85-0.95). The exponential cultures were then divided into two flasks and cells in one flask were grown without ethanol, whereas cells in the latter flask were treated with ethanol to have 8% (v/v) final ethanol concentration. In order to analyze the transcriptional response of each strain to ethanol, samples were collected 2 h after ethanol treatment from both treated and untreated cultures, immediately frozen in liquid nitrogen and stored at -80C until RNA isolation. All experiments were carried out in triplicate.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-3874
refinement:
raw
dateSubmitted:
01-07-2013
keywords:
functional genomics
dateModified:
12-11-2015
creators:
Ceyda Kasavi
availability:
available
types:
gene expression
name:
Saccharomyces cerevisiae
name:
stimulus or stress design
ID:
A-AFFY-47
name:
Affymetrix GeneChip Yeast Genome 2.0 Array [Yeast_2]
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3874/E-MTAB-3874.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3874/E-MTAB-3874.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress