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Title: Zebrafish larvae submitted to simulated microgravity on a clinostat (CLINO) at 5dpf for one day      
dateReleased:
02-15-2016
description:
The general objective of the study was to determine modulation of gene expression by environmental factors, specifically simulation of microgravity, with a special emphasis on bone formation. For this reason, the specific period of treatment was chosen between 5-6 days post-fertilization (dpf), when bone formation and calcification are taking place. Zebrafish larvae were placed at 5 dpf into a clinostat (CLINO) for 24 hours, which was shown to simulate microgravity by the specific rotational movements it generates. We show that CLINO exposure causes a clear decrease of bone formation, as illustrated by cranial skeleton staining of the bone matrix by Alizarin Red, by morphometric analysis of the resulting images. Thus, a whole genome micro-array experiment was conducted to identify genes that may be involved in the observed effect on bone formation.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-4476
refinement:
raw
dateSubmitted:
09-06-2008
keywords:
functional genomics
dateModified:
02-16-2016
availability:
available
types:
gene expression
name:
Danio rerio
ID:
A-AGIL-30
name:
Agilent D. rerio (Zebrafish) Oligo Microarray 013223 G2518A Option 001
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-4476/E-MTAB-4476.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-4476/E-MTAB-4476.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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