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Title: Expression data from differentiating mouse embryonic stem cells wild type and lacking functional Pax7 gene      
dateReleased:
02-26-2016
description:
Myogenic differentiation relies on Pax7 function. We used embryonic stem cells lacking functional Pax7 to follow its role in derivation of skeletal myoblasts. Microarray analysis allowed us to compare transcriptomes of undifferentiated and differentiating embryonic stem cells of two genotypes, i.e. Pax7+/+ and Pax7-/- at day 7 and 21 of culture. Undifferentiated mouse embryonic stem cells, embryiod bodies at day 7, and embryoid body outgrowths at day 21 of differentiation were collected for RNA extraction and hybridization on Affymetrix microarrays.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-66483
refinement:
raw
alternateIdentifiers:
66483
keywords:
functional genomics
dateModified:
03-07-2016
availability:
available
types:
gene expression
name:
Mus musculus
ID:
A-GEOD-17400
name:
[MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version]
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-66483/E-GEOD-66483.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-66483/E-GEOD-66483.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE66483
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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