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Title: Real-time Quantitative PCR analysis of the expression of TGFB-pathway target genes on 3D-cultured chemoresistant colon carcinoma cells      
dateReleased:
03-10-2016
description:
The aim of the study was to elucidate which TGFB signaling pathway molecules are involved in the colon cancer chemoresistance. The PCR array used for this experiments was 84 Human TGF-β Signaling Targets (Cat. N. PAHS-235ZA, Qiagen) The expression of 84 Human TGF-β Signaling Targets genes was analyzed by RT2 profiler PCR array (PAHS-235ZA, Qiagen) using the StepOne Plus instrument (Applied Biosystems) following the manufacturer’s protocol. Two independent experiments were performed for each group of treatment. Untreated cells were used as reference control sample. The mRNA expression levels of each gene in each cell treatment were normalized using the expression of the housekeeping genes B2M, GAPDH, RPLP0, HPRT1 and ACTB.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-77927
refinement:
raw
alternateIdentifiers:
77927
keywords:
functional genomics
dateModified:
03-20-2016
availability:
available
types:
gene expression
name:
Homo sapiens
ID:
A-GEOD-21455
name:
SA Biosciences RT² Profiler PCR Panel Human TGFb Signaling Targets
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-77927/E-GEOD-77927.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-77927/E-GEOD-77927.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE77927
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress
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