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Title: The effect of growth rate on pyrazinamide activity in Mycobacterium tuberculosis; insights for early bactericidal activity      
dateReleased:
03-25-2016
description:
Background: Pyrazinamide (PZA) plays an essential part in the shortened 6-month tuberculosis (TB) treatment course due to its activity against slow-growing, semi-dormant organisms. We tested the paradigm that PZA preferentially targets slow growing cells of Mycobacterium tuberculosis that remain after the initial kill by isoniazid, by observing the response of either slow growing or fast growing bacilli to differing concentrations of PZA. Methods: M. tuberculosis H37Rv was grown in continuous culture at either a constant fast growth rate (Mean Generation Time [MGT] of 23.1 h) or slow growth rate (69.3 h MGT) at a controlled dissolved oxygen tension of 10% and a controlled acidity at pH 6.3 ± 0.1. The cultures were exposed to step-wise increases in the concentration of PZA (25 µg ml-1 to 250 µg ml-1) every 2 MGTs, and bacterial survival was measured. PZA-induced global gene expression was explored for each increase in PZA-concentration, using microarray.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-4093
refinement:
raw
keywords:
functional genomics
dateModified:
12-02-2015
availability:
available
types:
gene expression
name:
Mycobacterium tuberculosis H37Rv
name:
growth condition design
ID:
A-BUGS-47
name:
BuGS M.tuberculosis 177440 TBvH37Rv 180k Tiling
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-4093/E-MTAB-4093.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-4093/E-MTAB-4093.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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