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Title: Genome-wide methylation profiles in primary intracranial germ cell tumors indicate primordial germ cell origin of germinoma [Agilent]      
dateReleased:
04-01-2016
description:
The pathogenesis of intracranial germ cell tumors (iGCTs) is not yet fully uncovered despite exhaustive genomic analyses. By means of a genome-wide methylation analysis, we show that pure germinoma is characterized by global DNA low methylation, a unique epigenetic feature distinct from all other subtypes of iGCTs. The patterns of methylation strongly resemble that of primordial germ cells (PGC) at the migration phase, indicating the cells of origin. Unlike PGC, however, hypomethylation extends to LINE1 retrotransposon. Microdissected histologically and epigenetically distinct components of mixed-GCTs shared identical mutations in the MAPK or PI3K pathways, suggesting that the genetic alterations were likely to have occurred at the pre-implantation phase in early embryogenesis. Thus, we propose iGCTs are “Zygotoma”. Genomic DNA from the 81 GCTs were hybridized to the SurePrint G3 Human CGH 8 × 60 K Oligo Microarrays G4450A (Agilent Technologies, Santa Clara, CA).
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-70782
refinement:
raw
alternateIdentifiers:
70782
keywords:
functional genomics
dateModified:
04-11-2016
availability:
available
types:
gene expression
name:
Homo sapiens
ID:
A-GEOD-17052
name:
Agilent-021924 SurePrint G3 Human CGH Microarray 8x60K (Probe Name version)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-70782/E-GEOD-70782.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-70782/E-GEOD-70782.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE70782
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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