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Title: Identifying genes dysregulated in patients with acute myocardial infarction      
dateReleased:
05-11-2016
description:
Twenty-three AMI patients and 23 non-AMI healthy controls were included in this pilot study. Tissue samples were cut at 10 μm from formalin fixed paraffin-embedded tissue blocks using a microtome. Six to eight 10-μm sections were used for the isolation procedure. Total RNA isolation was performed using a miRNeasy FFPE kit (Qiagen) according to the manufacturer’s protocol. RNA concentration and purity was determined and before gene expression profiling (Affymetrix Human Exon 1.0ST Array). The microarray labeling, hybridization and processing was performed according to the manufacturer’s protocol at the Microarray Core Facility of Chinese National Human Genome Center, Shanghai, China. The raw data of microarray were quantile-normalized over all samples, summarized with the robust multi-array average (RMA) algorithm and log2 transformed with a median polish for ~22,000 transcript clusters (gene-level). Significance Analysis of Microarrays (SAM) was used to identify differential genes between AMI patients and non-AMI controls. Please note that the data files in this experiment have been previously submittted to NCBI Gene Expression Omnibus under accession numbers GSE38958 and GSE49081 (imported as E-GEOD-38958 and E-GEOD-49081 respectively in ArrayExpress ).
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-3573
refinement:
raw
dateSubmitted:
11-27-2009
keywords:
functional genomics
dateModified:
07-08-2016
creators:
yaping wang
availability:
available
types:
gene expression
name:
Homo sapiens
name:
case control design
ID:
A-MEXP-2246
name:
Affymetrix GeneChip Human Exon 1.0 ST Array version 2, [HuEx-1_0-st-v2]
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3573/E-MTAB-3573.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3573/E-MTAB-3573.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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