Mountain View
biomedical and healthCAre Data Discovery Index Ecosystem
help Advanced Search
Title: Transcriptomic response of Rhizobium leguminosarum strain Rlv3841 to concentrated pea root exudate      
dateReleased:
05-25-2016
description:
The secretion of metabolites by plant roots is a key determinant of microbial growth and colonisation. We have used Pisum sativum and its natural symbiont Rhizobium leguminosarum (it can form N2 fixing nodules on pea roots) to study the natural metabolites secreted by roots. To do this root secretion was harvested from pea plants grown under sterile conditions. This root exudate was then concentrated and used as a sole carbon and nitrogen source for growth of the bacteria in the laboratory. These bacteria were harvested in mid-exponential growth and RNA extracted for microarray analysis. As control cultures the bacteria were grown on 30 mM pyruvate as a carbon source and 10 mM ammonium chloride as a nitrogen source and RNA extracted. Two colour microarrays were performed using root exudate cultures versus pyruvate ammonia grown cultures. This was done in biological triplicate.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-4790
refinement:
raw
dateSubmitted:
11-11-2011
keywords:
functional genomics
dateModified:
05-26-2016
creators:
philip Poole
availability:
available
types:
gene expression
name:
Rhizobium leguminosarum
name:
growth condition design
ID:
A-MTAB-591
name:
Agilent custom Rhizobium leguminosarum bv. viciae 3841 genome array, Rlv39841 (Agilent grid ID 030602)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-4790/E-MTAB-4790.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-4790/E-MTAB-4790.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on GitHub.