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Title: Transcription profiling by array of the HCT116 colorectal cancer cell line following naltrexone treatment      
dateReleased:
06-01-2016
description:
A number of reports have identified a putative anticancer role for naltrexone when used at doses lower than those conventionally administered. In particular, it has been shown that these low doses of naltrexone are able to suppress tumour growth. A definitive mechanism of action has yet to be established, but what is known is that the effect can be via modifications to the host immune system rendering it more anticancer in nature, or through direct antagonism of tumour growth. Our current paper uses gene expression as a way of unraveling this.The human colorectal cancer cell line HCT116 was cultured in medium alone, or with naltrexone at two different doses for 4 hours. Cells were either harvested after this time or transferred into fresh medium for a further 24 hours before being harvested. RNA was isolated from these cells by Trizol. Total RNA was labelled and hybridised to Illumina Human HT-12v4 arrays.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-4454
refinement:
raw
keywords:
functional genomics
dateModified:
04-25-2016
availability:
available
types:
gene expression
name:
Homo sapiens
ID:
A-MEXP-2072
name:
Illumina HumanHT-12_V4_0_R2_15002873_B
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-4454/E-MTAB-4454.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-4454/E-MTAB-4454.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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