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Title: Angiogenesis and Gene Signature in Muscular Hypertrophy      
dateReleased:
06-28-2016
description:
Adult myogenic progenitor cells (activated satellite cells) express both HGF and its receptor cMet. Following muscle injury, autocrine HGF-Met stimulation plays a key role in promoting activation and early division of satellite cells. Magic-F1 (Met-Activating Genetically Improved Chimeric Factor-1) is an HGF-derived, engineered protein that contains two Met-binding domains that promotes muscle hypertrophy, protecting myogenic precursors against apoptosis, increasing their fusion ability and enhancing muscle differentiation. Hemizygous and homozygous Magic-F1 transgenic mice displayed constitutive muscle hypertrophy. We described here microarray analysis on Magic-F1 myogenic progenitor cells showing an altered gene signatures involving muscle hypertrophy and vasculogenesis compared to wild-type cells. In parallel, we performed a functional analysis on homozygous Magic-F1 transgenic mice versus control, demonstrating that Magic-F1+/+ mice display impairment on running performance. Finally, we show that induced muscle hypertrophy affects positively vascular network, increasing vessel number in fast twitch fibers. This is due to unique features of mammalian skeletal muscle and its remarkable ability to adapt promptly to different physiological demands by changing gene expression profile. Biological triplicates of Magic-F1+/+ and control satellite cells were used for microarray analysis.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-83771
refinement:
raw
alternateIdentifiers:
83771
keywords:
functional genomics
dateModified:
07-03-2016
availability:
available
types:
gene expression
name:
Mus musculus
ID:
A-MEXP-724
name:
Agilent Whole Mouse Genome Microarray 4x44K 014868 G4122F (annotation from 02.2007)
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-83771/E-GEOD-83771.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-83771/E-GEOD-83771.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE83771
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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