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Title: Genome wide gene expression during lung development in three inbred mouse strains      
dateReleased:
07-01-2016
description:
To better understand the temporal dynamics of gene expression during normal murine lung development we characterized global gene expression at 26 time points in three common inbred strains of mice (A/J, C57BL/6J, and C3H/HeJ). The data set provides a unique resource for identifying patterns of gene expression changes during normal lung development and for investigating the developmental origins of respiratory disease. The transcriptional profiles generated for lung development in three inbred strains of the laboratory mouse revealed concordance with pre-natal stages of lung development defined by anatomy and morphology. The genomic data support the view that the postnatal alveolar development is composed of 4 distinct molecular stages. The data revealed strain specific differences in the expression of genes related to respiratory cell differentiation, pulmonary innervation, metabolic pathway interactions, and immune system function. Mouse lungs or whole embryos (e09.5 only) were surgically dissected at 26 time points across all six canonical stages of lung development (embryonic-EMB, pseudoglandular-PSG, canalicular-CAN, saccular-SAC, alveolar-ALV, homeostatic-HOM) to identify transcriptional profiles associated with 9 molecular divisions of lung development (embryonic-EMB, pseudoglandular-PSG, canalicular-CAN, saccular-SAC, alveolar1-ALV1, alveolar2-ALV2, alveolar3-ALV3, alveolar4-ALV4, homeostatic-HOM). Lung tissue from three or four embryos collected at e11.5 or e12.5, respectively, was pooled for each sample to obtain sufficient RNA for array analysis. Tissue from at least three animals was collected for each time point (biological replicates). Only male mice were used in the study.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-74243
refinement:
raw
alternateIdentifiers:
74243
keywords:
functional genomics
dateModified:
07-11-2016
availability:
available
types:
gene expression
name:
Mus musculus
ID:
A-GEOD-20894
name:
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [CDF: MoGene10stv1_Mm_ENSG v.19.0.0]
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-74243/E-GEOD-74243.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-74243/E-GEOD-74243.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE74243
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress