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Title: Transcription profiling of hearts and kidneys of spontaneously hypertensive rat and coisogenic rat strain with connexin50 mutation      
dateReleased:
07-01-2016
description:
We aimed to compare transcriptome in heart and livers of spontaneously hypertensive rat SHR/OlaIpcv (Rat Genome Database ID: 631848) versus coisogenic rat strain SHR-Gja8m1Cub (Rat Genome Database ID: 2293729) using Affymetrix GeneChip® Rat Exon 1.0 ST Arrays in triplicate. Affymetrix GeneChip® Rat Exon 1.0 ST Array was used to determine the transcriptomic characteristics of the SHR and the coisogenic strain SHR-Dca. We extracted total RNA from the kidneys and hearts of 4-month old males of both strains using phenol-chloroform and purified with the RNeasy Mini kit (Qiagen, Valencia, CA, USA) in accordance with the manufacturer’s protocol. The quality of the total RNA was verified by the Agilent 2100 Bioanalyzer system. Double-stranded complementary DNA (cDNA) was synthesized from total RNA. The labeled and fragmented cDNA was pooled for each strain (SHR, SHR-Dca) and tissue (heart, kidney) and hybridized to Affymetrix GeneChip® Rat Exon 1.0 ST Arrays in triplicate (total 12 arrays). The whole hybridization procedure including DNA adjustment was performed according to the protocol recommended by Affymetrix.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-4542
refinement:
raw
dateSubmitted:
06-03-2009
keywords:
functional genomics
dateModified:
03-04-2016
creators:
Ondřej Šeda
availability:
available
types:
gene expression
name:
Rattus norvegicus
name:
strain or line design
ID:
A-AFFY-160
name:
Affymetrix GeneChip Rat Gene 1.0 ST Array [RaGene-1_0-st-v1]
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-4542/E-MTAB-4542.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-4542/E-MTAB-4542.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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