Mountain View
biomedical and healthCAre Data Discovery Index Ecosystem
help Advanced Search
Title: Interferon-stimulated gene expression in STAT1 knockout cells      
dateReleased:
07-22-2016
description:
STAT1 plays a cental role in the induction of interferon-stimulated genes, but interferon alpha can activate a STAT1-independent pathway that leads to gene expression. We performed microarray analysis to examine whether like interferon alpha, interferon lambda, a newly discovered interferon, can induce the expression of interferon-stimulated genes in the absence of STAT1. Control and STAT1 knockout Huh-7.5 hepatoma cells were left untreated or treated with 1000 U/ml of human interferon alpha 2a or interferon lambda 1 (PBL) for 24 h, and total RNA was extracted. Sense-strand DNA was generated from 200 ng of total RNA, fragmented, and labeled using a GeneChip WT Plus Reagent Kit (Affymetrix). Six samples were obtained and each sample was anlyzed using one GeneChip.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-84693
refinement:
raw
alternateIdentifiers:
84693
keywords:
functional genomics
dateModified:
07-31-2016
availability:
available
types:
gene expression
name:
Homo sapiens
ID:
A-GEOD-16686
name:
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version]
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-84693/E-GEOD-84693.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-84693/E-GEOD-84693.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE84693
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress