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Title: Conversion of Human Somatic Cells to Multipotent Endoderm Stem Cells by Small Molecules [array]      
dateReleased:
07-24-2016
description:
Forced expression of transcription factors for lineage reprogramming brings hope to cell-based therapy. However, its application is hampered by risks of potential genetic aberrations and tumorigenicity. Using defined small molecules in presence of gastric stromal cells as feeders, we reprogramed human gastric epithelia into induced multipotent endodermal progenitors (hiMEPs) with efficiency of up-to-6%. The hiMEPs expressed genes relative to endodermal lineages but not associating with pluripotency, and could be expanded clonogenically remaining as undifferentiated colonies. Upon induction, hiMEPs were able to give rise to multiple functional endodermal cell types, apart from ectodermal or mesodermal lineages. TGFβ inhibition and particular Wnt signaling activation were crucial in reprogramming process. Collective advantages of availability from donors without age restriction, capabilities in expansion and differentiation, and no concern of tumorigenesis, let hiMEPs have the considerable application potentials on cell therapies of diseases such as liver failure and diabetes, as well as personalized drug-screenings. Human gastric epithelial cells (hGECs) and gastric subepithelial myofibroblasts (GSEMFs) are isolated from human stomach, and human duodenum epithelial cells (hDECs) are isolated from human duodenum. Human Induced multipotent endodermal stem cells (hiMESCs) were reprogrammed from hGECs/hDECs by small molecules with the support of GSEMFs. Definitive endoderm cells (DEs) are derived from human embryonic stem cells by differentiation. Totally, 13 samples including two samples of hDECs, one sample of hGECs, two clones of D-hiMESCs, one clone of G-hiMESCs, four samples of DEs and three samples of GSCs were analyzed using microarray.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-71301
refinement:
raw
alternateIdentifiers:
71301
keywords:
functional genomics
dateModified:
07-30-2016
availability:
available
types:
gene expression
name:
Homo sapiens
ID:
A-GEOD-10558
name:
Illumina HumanHT-12 V4.0 expression beadchip
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-71301/E-GEOD-71301.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-71301/E-GEOD-71301.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE71301
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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