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Title: Biological function of antisense RNAs that are synthesized by RNA-dependent RNA polymerases under abiotic stress      
dateReleased:
07-31-2016
description:
To identify RDR1/2/6-dependent antisense RNA loci under 2hr drought stress, expression profiles of Poly (A+) RNA and Poly (A-) RNA between rdr1/2/6 and wild-type using the custom microarray (GPL19830) were analyzed. rdr1/2/6 and wild-type plants (Arabidopsis thaliana ecotype Columbia) were grown on MS medium for 2 hours. Then, the plants were transferred to plastic dish and kept for 1 week. Poly (A+) RNA and Poly (A-) fractions were separated from 100μg total RNA by treating with Ambion Poly A purist twice. Poly (A-) RNA sample was prepared by depleting rRNA using Invitrogen RiboMinus Plant Kit. 5 ng of Poly (A+) RNA and 1.4 μg of Poly (A-) RNA samples were used for the expression profiling using the custom array (GPL19830).
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-GEOD-72309
refinement:
raw
alternateIdentifiers:
72309
keywords:
functional genomics
dateModified:
08-07-2016
availability:
available
types:
gene expression
name:
Arabidopsis thaliana
ID:
A-GEOD-20796
name:
Agilent-034592 Arabidopsis thaliana PSC_Custom
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-72309/E-GEOD-72309.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-72309/E-GEOD-72309.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE72309
storedIn:
Gene Expression Omnibus
qualifier:
not compressed
format:
HTML
accessType:
landing page
primary:
true
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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