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Title: Relating differences in the colonisation potential of verotoxigenic Escherichia coli (VTEC) for plant hosts to wholescale genes in gene expression      
dateReleased:
08-01-2016
description:
Verotoxigenic Escherichia coli (VTEC) are a leading cause of food-borne illness. Fruit and vegetables are recognised as an important source of the pathogen and can account for ~ 25 % of food-borne VTEC outbreaks, globally. The ability of VTEC to colonise leaves and roots of leafy vegetables, spinach (Spinacia oleracea) and lettuce (Lactuca sativa), was compared. The highest levels of colonisation occurred in the roots and rhizosphere, whereas colonisation of the leaves was lower and significantly different between the species. Colonisation of the leaves of prickly lettuce (L. serriola), a wild relative of domesticated lettuce, was especially poor. Differential VTEC gene expression in spinach extracts was markedly different for three tissue types, with little overlap. Comparison of expression in the same tissue type, cell wall polysaccharides, for lettuce and spinach also showed substantial differences, again with virtually no overlap. The transcriptional response was largely dependent on temperatures that are relevant to plant growth, not warm-blooded animals. The data show that VTEC adaptation to plant hosts and subsequent colonisation potential is underpinned by wholescale changes in gene expression that are specific to both plant tissue type and to the species.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-3249
refinement:
raw
dateSubmitted:
01-09-2015
keywords:
functional genomics
dateModified:
03-02-2016
creators:
Pete E Hedley
availability:
available
types:
gene expression
name:
Escherichia coli O157:H7 str. Sakai
ID:
A-GEOD-8701
name:
Agilent Escherichia coli 15K array
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3249/E-MTAB-3249.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-3249/E-MTAB-3249.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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