Title: | A novel tumor-associated myeloid cell population inhibits antigen-specific immune responses in cancer patients
![]() |
dateReleased: |
08-04-2016
|
description: |
Tumor progression is associated with an immunosuppressive microenvironment that consists of several elements, such as regulatory T cells, type 2 macrophages and myeloid-derived suppressor cells. Here, we identify for the first time a BDCA1+CD14+ population of immunosuppressive cells that resides both in the blood and tumor of melanoma patients. We demonstrated that the presence of these cells in dendritic cell (DC)-based anti-tumor vaccines significantly suppresses CD4+ T cells in an antigen-specific manner. In an attempt to reveal the mechanism of this suppressive activity, we noticed that BDCA1+CD14+ cells express elevated levels of the check-point molecule PD-L1, which thereby hinders T cell proliferation. Importantly, although this suppressive BDCA1+CD14+ population expresses markers of both BDCA1+ DCs and monocytes, functional, transcriptome and proteome analyses clearly revealed that they comprise a unique population of cells that is exploited by tumors to evade immunity. Thus, targeting these cells may improve the efficacy of cancer immunotherapy. mRNA profiles of BDCA1+ DCs, BDCA1+CD14+ cells and monocytes, isolated from 3 healthy volunteers, were generated by deep RNA sequencing using HiSeq 2000 System (TruSeq SBS KIT-HS V3,Illumina)
|
privacy: |
not applicable
|
aggregation: |
instance of dataset
|
ID: |
E-GEOD-75042
|
refinement: |
raw
|
alternateIdentifiers: |
75042
|
keywords: |
functional genomics
|
dateModified: |
08-05-2016
|
availability: |
available
|
types: |
gene expression
|
name: |
Homo sapiens
|
accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-75042/E-GEOD-75042.raw.1.zip![]() |
storedIn: |
ArrayExpress
|
qualifier: |
gzip compressed
|
format: |
TXT
|
accessType: |
download
|
authentication: |
none
|
authorization: |
none
|
accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-75042/E-GEOD-75042.processed.1.zip![]() |
storedIn: |
ArrayExpress
|
qualifier: |
gzip compressed
|
format: |
TXT
|
accessType: |
download
|
authentication: |
none
|
authorization: |
none
|
accessURL: | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE75042 |
storedIn: |
Gene Expression Omnibus
|
qualifier: |
not compressed
|
format: |
HTML
|
accessType: |
landing page
|
primary: |
true
|
authentication: |
none
|
authorization: |
none
|
abbreviation: |
EBI
|
homePage: | http://www.ebi.ac.uk/ |
ID: |
SCR:004727
|
name: |
European Bioinformatics Institute
|
homePage: | https://www.ebi.ac.uk/arrayexpress/ |
ID: |
SCR:002964
|
name: |
ArrayExpress
|