biomedical and healthCAre Data Discovery Index Ecosystem
biomedical and healthCAre Data Discovery Index Ecosystem
| Title: | ZIP7 is indispensable for proliferation of intestinal epithelial cells and maintenance of intestinal stem cells
|
| dateReleased: |
08-19-2016
|
| description: |
Intestinal epithelium are generated by intestinal stem cells, which are recognized morphologically as slender columnar cells at the base of the crypt. Stem cells produce transit-amplifying (TA) cells, which divide a number of times and the daughter cells differentiate into absorptive enterocytes as well as secretory-lineages. Intestinal stem cells highly express Lgr5 which is decreased in TA cells. Here, we show that the zinc transported SLC39A7/ZIP7 is essential for the proliferation of TA cells and maintenance of intestinal stem cells. Lgr5Med TA cells derived from Zip7-deficient mice upregulated the expression of unfold protein responses-related genes including pro-apoptotic genes, indicating of induction of ER stress in these cells. The same effect was seen in Lgr5Hi stem cells derived from Zip7-deficient mice. We conclude that ZIP7 is fundamental to the maintenance of crypt homeostasis by resolving ER stress. Small intestinal crypts were isolated form tamoxifen-treated control (Zip7flox/+, Villin-CreERT2, Lgr5-EGFP-ires-CreERT2) and tamoxifen-treated Zip7∆IEC (Zip7flox/flox, Villin-CreERT2, Lgr5-EGFP-ires-CreERT2) mice. We FACS purified intestinal crypt cells according to their Lgr5 expression levels. RNA was isolated from four FACS sorted cell populations: Lgr5Hi cells and Lgr5Med cells derived from control mice, Lgr5Hi cells and Lgr5Med cells derived from Zip7∆IEC mice. Isolated RNA was analyzed using the Affymetrix platform.
|
| privacy: |
not applicable
|
| aggregation: |
instance of dataset
|
| ID: |
E-GEOD-72190
|
| refinement: |
raw
|
| alternateIdentifiers: |
72190
|
| keywords: |
functional genomics
|
| dateModified: |
09-12-2016
|
| availability: |
available
|
| types: |
gene expression
|
| name: |
Mus musculus
|
| ID: |
A-AFFY-130
|
| name: |
Affymetrix GeneChip Mouse Gene 1.0 ST Array [MoGene-1_0-st-v1]
|
| accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-72190/E-GEOD-72190.raw.1.zip |
| storedIn: |
ArrayExpress
|
| qualifier: |
gzip compressed
|
| format: |
TXT
|
| accessType: |
download
|
| authentication: |
none
|
| authorization: |
none
|
| accessURL: | https://www.ebi.ac.uk/arrayexpress/files/E-GEOD-72190/E-GEOD-72190.processed.1.zip |
| storedIn: |
ArrayExpress
|
| qualifier: |
gzip compressed
|
| format: |
TXT
|
| accessType: |
download
|
| authentication: |
none
|
| authorization: |
none
|
| accessURL: | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE72190 |
| storedIn: |
Gene Expression Omnibus
|
| qualifier: |
not compressed
|
| format: |
HTML
|
| accessType: |
landing page
|
| primary: |
true
|
| authentication: |
none
|
| authorization: |
none
|
| abbreviation: |
EBI
|
| homePage: | http://www.ebi.ac.uk/ |
| ID: |
SCR:004727
|
| name: |
European Bioinformatics Institute
|
| homePage: | https://www.ebi.ac.uk/arrayexpress/ |
| ID: |
SCR:002964
|
| name: |
ArrayExpress
|