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Title: Transcription profiling by array of human primary astrocytes, cancer stem cells derived from astrocytes overexpressing various oncogenic and iPSC-inducing factors, glioma stem cells and gliblastomat cell line to compare their expression profiles and tumor-initiating capabilities      
dateReleased:
11-07-2016
description:
Cancer stem cells play key roles in human malignancies. However, their cellular and molecular origins are still poorly understood. Here we report the successful generation of cancer stem cells from primary human astrocytes by use of defined genetic factors. Combined transduction of four factors —myc, Oct-4, p53DD, and ras— induced efficient transformation of primary human astrocytes into malignant cells with powerful tumor-initiating capabilities. Transplantation of 100 of the cells into nude mice is sufficient for tumor formation. The cells also showed unlimited self-renewal ability with robust telomerase activities. In addition, they expressed typical glioma stem cell markers such as CD133, CD15, and CD90. Moreover, transformed cells could form spheres in culture and differentiate into neuron-astrocyte-, and oligodendrocyte-like cells. They also displayed significant resistance to temozolomide. These induced cancer stem cells (iCSCs) should be useful for both tumor biology studies and therapeutics development. This record contains data from microarray analysis of normal human astrocytes, two transduced cell lines (OCT4+3G and OCT4+6G), a patient-derived glioma stem cell line (ALPS1459) and a glioblastoma cell line (U87MG).
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-4771
refinement:
raw
dateSubmitted:
09-16-2013
keywords:
functional genomics
dateModified:
11-07-2016
availability:
available
types:
gene expression
name:
Homo sapiens
name:
cell type comparison design
ID:
A-AFFY-37
name:
Affymetrix GeneChip Human Genome U133A 2.0 [HG-U133A_2]
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-4771/E-MTAB-4771.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-4771/E-MTAB-4771.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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