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Title: Transcriptomic characterization of cattle primary cultured hepatocytes and its comparison with liver tissue      
dateReleased:
01-31-2017
description:
Cattle primary cultured hepatocytes (CHs) have been used as a useful in vitro model for drug metabolism studies. However, they suffer some limitations, when compared with the ex vivo liver tissue, such as difficult adhesion to the substrate following isolation, a limited viability and a rapid loss of differentiated characteristics. Therefore, a reliable hepatic in vitro model is actually needed for cattle. As established bovine hepatic cell lines are not available, we characterized the cattle primary CHs' transcriptome by using a cDNA microarray approach and compared it with ex vivo cattle liver tissue as well as with the Madin-Darby Bovine Kidney (MDBK) cells (as an established cell line for transfection purposes).This part of the study compares the primary CHs vs. liver tissue. The comparison of MDBK cells vs. the primary CHs is found under E-MTAB-4430.
privacy:
not applicable
aggregation:
instance of dataset
ID:
E-MTAB-4424
refinement:
raw
dateSubmitted:
01-01-2015
keywords:
functional genomics
dateModified:
02-09-2017
creators:
Ramy Elgendy
availability:
available
types:
gene expression
name:
Bos taurus
ID:
A-MEXP-1810
name:
Agilent-015354 Bovine Oligo Microarray
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-4424/E-MTAB-4424.raw.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
accessURL: https://www.ebi.ac.uk/arrayexpress/files/E-MTAB-4424/E-MTAB-4424.processed.1.zip
storedIn:
ArrayExpress
qualifier:
gzip compressed
format:
TXT
accessType:
download
authentication:
none
authorization:
none
abbreviation:
EBI
homePage: http://www.ebi.ac.uk/
ID:
SCR:004727
name:
European Bioinformatics Institute
homePage: https://www.ebi.ac.uk/arrayexpress/
ID:
SCR:002964
name:
ArrayExpress

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